Purification and characterization of the overexpressed Aspergillus oryzae xylanase, XynF1

Authors
Kitamoto, N Yoshino, S Ohmiya, K Tsukagoshi, N
Citation
N. Kitamoto et al., Purification and characterization of the overexpressed Aspergillus oryzae xylanase, XynF1, BIOS BIOT B, 63(10), 1999, pp. 1791-1794
Citations number
10
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
09168451 → ACNP
Volume
63
Issue
10
Year of publication
1999
Pages
1791 - 1794
Database
ISI
SICI code
0916-8451(199910)63:10<1791:PACOTO>2.0.ZU;2-F
Abstract
The Aspergillus oryzae xynF1 gene coding for a xylanase, XynF1, was success fully overexpressed under the strong A. oryzae TEF1 gene promoter. The high -XynF1-producing transformant secreted about 180 mg/l of XynF1 in the gluco se-containing medium. The overexpressed XynF1 was purified by only one chro matographic step. The purified XynF1 had a molecular mass of 35.0 kDa, a pH optimum of 5.0, and a temperature optimum of 60 degrees C.