Expression of BDNF and TrkB mRNAs in the crista neurosensory epithelium and vestibular ganglia following ototoxic damage

Following ototoxic lesion with the aminoglycoside gentamicin, the vestibula r neurosensory epithelia undergo degeneration and then Limited spontaneous regeneration. The spatio-temporal expression of brain-derived neurotrophic factor (BDNF) and of its high affinity receptor (trkB) mRNA was investigate d in the vestibular end organs and ganglia of chinchillas following gentami cin ototoxicity. In the vestibular ganglia of untreated chinchillas, the le vel of expression of BDNF mRNA is low. At 1 and 2 weeks after intraotic tre atment with gentamicin, BDNF mRNA levels in the vestibular ganglia were ele vated significantly compared to untreated chinchillas and chinchillas 4 wee ks after treatment. At 4 weeks after gentamicin treatment, BDNF mRNA levels were at intact levels of expression. In the crista ampullaris, high levels of BDNF transcripts were found in the untreated chinchillas. At 1 and 2 we eks after treatment, when only supporting cells are present in the crista, BDNF mRNA was undetectable. Four weeks after aminoglycoside treatment BDNF mRNA was present in the epithelium but at lower levels than in the intact e pithelium. In contrast to its ligand, high levels of trkB mRNA hybridizatio n were present in the vestibular ganglia of untreated chinchillas and trkB mRNA levels did not change following gentamicin treatment. In the vestibula r epithelia, trkB mRNA was not detected either in the intact epithelium or after gentamicin ototoxicity. These data suggest that BDNF may be involved in the maintenance of the vestibular ganglia and contribute to neurite outg rowth to new and repaired hair cells following ototoxic damage. (C) 1999 El sevier Science B.V. All rights reserved.