The literature on sequencing as a tool for yeast molecular taxonomy is revi
ewed. Ribosomal DNA has been preferred for sequencing over other molecules
such as mitochondrial DNA, and a large database is now available. rDNA cons
ists of regions that evolve at different rates, allowing comparison of diff
erent levels of relationship among yeasts. Sequences of the 18S rDNA and th
e 25S rDNA have been largely used for yeast systematics and phylogeny, but
the search for regions with increased resolving power has led to the study
of the spacer regions of the rDNA. Few studies are concerned with signature
sequences.