Cytosolic fatty acid binding protein enhances rat hepatocyte [H-3]palmitate uptake

Liver cytosolic fatty acid binding protein (FABP) represents the intracellu lar equivalent to extracellular serum albumin, participating in the intrace llular transport of long-chain fatty acids. In this study we observed the e ffect of increasing and decreasing FABP levels on hepatocyte [H-3]palmitate uptake in male Sprague-Dawley rats. We also were interested to determine w hether uptake, from either the unbound or unbound and protein-bound fractio ns, was fundamentally different at the different FABP levels. FABP levels w ere modified by hypophysectomy and clofibrate treatment (50 mg/100 g body w eight for 10 days). Results showed that the [H-3]palmitate clearance rates paralleled the 54% decrease and 73% increase in FABP levels in hypophysecto my and clofibrate-treated animals, respectively. In the presence of 2 and 2 0 mu M albumin, hepatocyte clearance rates of unbound [H-3]palmitate from h ypophysectomized animals (0.16 +/- 0.01 and 0.64 +/- 0.01 mL.s(-1).10(-6) c ells, respectively) were significantly lower (p < 0.01) than those of the s ham group (0.30 +/- 0.02 and 1.00 +/- 0.06 mL.s(-1).10(-6) cells, respectiv ely). However, the unbound [H-3]palmitate clearance rates from the clofibra te-treated group (0.39 +/- 0.04 and 1.18 +/- 0.12 mL.s(-1).10(-6) cells) we re significantly higher (p < 0.01) than the control group (0.29 +/- 0.02 an d 0.81 +/- 0.05 mL.s(-1).10(-6) cells) for 2 and 20 mu M albumin, respectiv ely. To investigate whether uptake was fundamentally different between the hypophysectomized and clofibrate-treated groups, we expressed the clearance rates as enhancement factors, i.e., EF = CL20 mu M/CL2 mu M. No statistica l difference was observed between EF of the hypophsectomized (3.8 +/- 0.4) and EF of the clofibrate-treated (3.1 +/- 0.3) groups, suggesting that the extracted ligand originated from similar fractions.