Microsatellite instability in hyperplastic and adenomatous polyps of the stomach

BACKGROUND. Few studies have focused on the presence and significance of mi crosatellite instability (MSI) in gastric polyps, and the results on record are conflicting. The aim of the current study was to address this issue, l aking into consideration the 2 main types of gastric polyps, the coexistenc e of foci of malignant transformation, and the expression of p53 and ERBB-2 . METHODS. Six hyperplastic polyps, 10 adenomatous polyps, and 4 adenomatous polyps displaying foci of malignant transformation (intestinal-type carcino ma) were studied for MSI. The authors analyzed a mononucleotide repeal micr osatellite (BAT-26) and 5 dinucleotide repeats in microdissected formalin f ixed, paraffin embedded tissue sections that were representative of the les ions. Expression of p53 and ERBB-2 were evaluated by immunohistochemistry. RESULTS. BAT-26 positivity was detected in 1 of 6 hyperplastic polyps (16.7 %) and in 2 of 10 adenomas (20%) without malignant transformation. In the 4 adenomatous polyps with carcinomatous foci, BAT-26 positivity was detected in 2 cases (50%) in both (adenomatous and carcinomatous) components of the lesions. p53 immunoreactivity was observed in 6 adenomatous polyps, 2 of t hem with malignant transformation, Overexpression of the ERBB-2 protein was detected in 1 adenomatous polyp with malignant transformation. CONCLUSIONS. Replication error (RER+) phenotype occurs in both hyperplastic and adenomatous polyps of the stomach. The highest frequency is observed i n adenomatous polyps with carcinomatous foci, suggesting that MSI may play a role in the process of malignant transformation in this setting. No signi ficant association was observed between RER+ phenotype and overexpression o f p53 or ERBB-2 proteins. (C) 1999 American Cancer Society.