Dot-ELISA for detection of Salmonella enterotoxin

Nineteen strains of Salmonella enterica belonging to seven serovars and two Strains of S. bongori were investigated for enterotoxin production by the Chinese hamster ovary (CHO) cell assay and Dot-ELISA. All the 19 strains we re found to carry stn gene when tested by polymerase chain reaction (PCR) u sing specific primers for stn sequence. Thirteen strains (68.42%) were foun d to produce Salmonella enterotoxin (Stn) when tested by the CHO cell assay . Dot-ELISA could detect Stn in 16 strains (84.21%). Dot-ELISA may be prefe rred over other assays due to its low cost and simplicity in performance.