R. Talon et al., Effect of nitrate and incubation conditions on the production of catalase and nitrate reductase by staphylococci, INT J F MIC, 52(1-2), 1999, pp. 47-56
The objective of this work was to study the production of catalase and nitr
ate reductase by staphylococci in order to understand their role in lipid o
xidation during sausage manufacturing. Catalase and nitrate reductase were
measured in resting cells and supernatants of staphylococci grown in differ
ent conditions. All staphylococci (except S. warneri) synthetized nitrate r
eductase. In static condition, the synthesis was maximal during exponential
,growth phase, whereas in shaking condition, the synthesis was maximal at t
he beginning of stationary phase. The production of nitrate reductase was i
ncreased in presence of nitrate, this effect was particularly important for
the two S. carnosus strains which exhibited the highest activity. For all
staphylococci, the production of catalase was maximal at the end of the exp
onential growth phase. The lowest amount of catalase was produced by S. war
neri and the highest by S. carnosus. Only S. xylosus 873 and S. saprophytic
us 852 released high amounts of catalase in the supernatant growth. Staphyl
ococci produced higher amounts of catalase in shaking conditions. Addition
of nitrate in the growth media favoured the synthesis of catalase, with a p
ronounced effect for S. carnosus. Nitrate also favoured the release of cata
lase. (C) 1999 Elsevier Science B.V. All rights reserved.