Detection and quantification of Salmonella in pure cultures using 5 '-nuclease polymerase chain reaction

Advances in detection and quantification assays based on nucleic acids conc eivably will revolutionize the ability to quickly and specifically detect a nd quantify microorganisms in foods. Among these assays, the polymerase cha in reaction (PCR) assay and the TaqMan(TM) PCR Detection System (Perkin-Elm er) probably lire among the most promising. Since a 5'-nuclease PCR renders possible the automated and direct detection and quantification of PCR prod ucts (Holland et al., 1991. Proc. Natl. Acad. Sci. USA 88, 7276-7280), micr oorganisms in foods can be detected and quantified indirectly within a few hours through analysis of the microbial DNA or RNA sequences present. In th e present report we have adapted a 5'-nuclease-based kit for the quantifica tion of Salmonella. (C) 1999 Elsevier Science B.V. All rights reserved.