Sensitivity of shuttle vector plasmid pZ189 DNA to carbon ion beams

Authors
Harada, K Ueda, T Ando, M Sawada, S Yamanaka, H Tamura, Y Watanabe, H Nagaoka, S Nakano, T Mukai, C Ohnishi, T
Citation
K. Harada et al., Sensitivity of shuttle vector plasmid pZ189 DNA to carbon ion beams, INT J MOL M, 4(5), 1999, pp. 509-511
Citations number
16
Categorie Soggetti
Medical Research General Topics
Journal title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN journal
11073756 → ACNP
Volume
4
Issue
5
Year of publication
1999
Pages
509 - 511
Database
ISI
SICI code
1107-3756(199911)4:5<509:SOSVPP>2.0.ZU;2-K
Abstract
The sensitivity of shuttle vector plasmid pZ189 DNA to carbon ion beams was studied under dry conditions. This plasmid contains the supF gene that can be used in mutagenesis analysis. The survival curve of the plasmid had bot h a shoulder and exponential parts, and showed a D-10 value of about 17 kGy for both Escherichia coli wild-type and DNA repair-deficient mutant (UV-se nsitive) strains. Therefore, it was assumed that the excision repair system was not effective for repairing DNA lesions induced by irradiation with ca rbon ion beams.