The major acidic glycolipids from the kidney of the pacific salmon (Oncorhynchus keta): Characterization of a novel ganglioside, fucosyl-N-acetylgalactosaminyl-GM1

Two fractions of a major ganglioside from the kidney of the pacific salmon, Oncorhynchus keta, mere eluted from a DEAE-Sephadex column in the monosial osyl fraction. The faster moving ganglioside (X1) on TLC- was separated fro m the slower moving one (X2) by HPLC using a silica beads column, By methyl ation analysis, chemical and enzymatic degradation, reaction with monoclona l antibodies, LSIMS, and H-1-NMR spectroscopy, X1 was determined to be a mo nosialosyl ganglioside belonging to the ganglio-series with a unique Fuc al pha 1-3GalNAc linkage at the nonreducing terminal: Fuc alpha 1-3GalNAc beta 1-3Gal beta 1-3GalNAc beta 1-4 [NeuAc alpha 2-3] G al beta 1-4Glc beta 1-1Cer Analysis of the lipophilic moiety indicated predominance of 24:1 fatty acid in combination with sphingenine. X2 was found to have a glycon structure i dentical to X1, The ceramide of X2 consisted predominantly of saturated fat ty acids (18:0 and 16:0), The tissue concentrations of X1 and X2 in kidney were 3.7 and 2.8 nmol/g, respectively.