Characterisation of starch from inner and peripheral parts of normal and waxy barley kernels

A previous microscopical examination of barley kernels indicated an uneven distribution of amylose in the waxy cultivar SW7142-92, where the subaleuro ne layer was strongly stained by iodine. To explain these results, starch w as isolated from fractions of inner and peripheral parts of kernels of the waxy cultivar and a cultivar with normal starch. Analyses of amylose conten t showed a higher concentration of amylose in starch from the outer part of the waxy barley kernel, where the amylose, determined by a colorimetric me thod, made up 8.6% of the starch compared with 5.9% in the middle and 2.2% in the innermost part of the waxy kernel. This difference in amylose distri bution was not observed in the normal starch kernel. The results were confi rmed by gel permeation chromatography. No differences in amylopectin chain length distribution were detected by size exclusion HPLC and HPAEC-PAD of a mylopectins from different parts of the barley kernels. (C) 1999 Academic P ress.