Nitric oxide (NO) inhalation has been reported to increase the oxygen affin
ity of sickle cell erythrocytes. Also, proposed allosteric mechanisms for h
emoglobin, based on S-nitrosation of P-chain cysteine 93, raise the possibi
lty of altering the pathophysiology of sickle cell disease by inhibiting po
lymerization or by increasing NO delivery to the tissue. We studied the eff
ects of a 2-hour treatment, using varying concentrations of inhaled NO. Oxy
gen affinity, as measured by P-50, did not respond to inhaled NO, either in
controls or in individuals with sickle cell disease. At baseline, the arte
rial and venous levels of nitrosylated hemoglobin were not significantly di
fferent, but NO inhalation led to a dose-dependent increase in mean nitrosy
lated hemoglobin, and at the highest dosage, a significant arterial-venous
difference emerged. The levels of nitrosylated hemoglobin are tao low to af
fect overall hemoglobin oxygen affinity, but augmented NO transport to the
microvasculature seems a promising strategy for improving microvascular per
fusion.