House dust mite (HDM) allergens are important factors in the increasing pre
valence of asthma. The lung epithelium forms a barrier that allergens must
cross before they can cause sensitization. However, the mechanisms involved
are unknown. Here we show that the cysteine proteinase allergen Der p 1 fr
om fecal pellets of the HDM Dermatophagoides pteronyssinus causes disruptio
n of intercellular tight junctions (TJs), which are the principal component
s of the epithelial paracellular permeability barrier. In confluent airway
epithelial cells, Der p 1 led to cleavage of the TJ adhesion protein occlud
in. Cleavage was attenuated by antipain, but not by inhibitors of serine, a
spartic, or matrix metalloproteinases. Putative Der p 1 cleavage sites were
found in peptides from an extracellular domain of occludin and in the TJ a
dhesion protein claudin-1. TJ breakdown nonspecifically increased epithelia
l permeability, allowing Der p 1 to cross the epithelial barrier. Thus, tra
nsepithelial movement of Der p 1 to dendritic antigen-presenting cells via
the paracellular pathway may be promoted by the allergen's own proteolytic
activity. These results suggest that opening of TJs by environmental protei
nases may be the initial step in the development of asthma to a variety of
allergens.