Ultraviolet-B radiation stimulates shikimate pathway-dependent accumulation of mycosporine-like amino acids in the coral Stylophora pistillata despite decreases in its population of symbiotic dinoflagellates

Colonies of Stylophora pistillata maintained for four years in indoor aquar ia in the near absence of ultraviolet radiation (UVR) contained only small amounts (<5 nmol mg(-1) protein) of 10 identified mycosporine-like amino ac ids (MAAs, which act as UV sunscreens), the largest number reported in any organism. The concentrations of most MAAs increased linearly or exponential ly when colonies were exposed to ultraviolet-A (UVA) and ultraviolet-B (UVB ) for 8 h d(-1) in the presence of photosynthetically active radiation (PAR ). Total MAA concentration reached 174 nmol mg(-1) protein after 30 d, with palythine and mycosporine-2 glycine constituting more than half of the fin al total. WE specifically stimulated MAA accumulation: after 15 d, MAA leve ls in colonies exposed to PAR alone and to PAR and WA did not differ (7 and 5 nmol MAA mg(-1) protein, respectively), while those in colonies exposed to PAR and UVA + UVB were significantly higher (28 nmol mg(-1) protein). Gl yphosate, an inhibitor of the shikimate pathway, eliminated or reduced the UV-induced accumulation of most MAAs during 7 d of exposure, providing the first experimental evidence of their synthesis via this pathway in a coral symbiosis. Densities of zooxanthellae in colonies of S. pistillata, Acropor a sp., and Seriatopora hystrix exposed to UVR for 15 d were only one-third of those in control colonies unexposed to UVR. This net decrease in the num ber of zooxanthellae in the corals (bleaching) occurred despite W-stimulate d increases in algal cytokinesis and in the host cell-specific density of z ooxanthellae in hospite, increases that apparently destabilized the symbios is and caused expulsion of the zooxanthellae.