J. Jaruzelska et al., Is selection responsible for the low level of variation in the last intronof the ZFY locus?, MOL BIOL EV, 16(11), 1999, pp. 1633-1640
DNA variability was investigated in the last intron of the Y-chromosome-spe
cific zinc finger gene, ZFY, and its X homolog on Xp21.3, ZFX. No polymorph
isms were found in the 676-bp ZFY segment in a sample of 205 worldwide-dist
ributed Y chromosomes, other than a solitary nucleotide variant in one indi
vidual (nucleotide diversity rr = 0.0014%). In contrast, 10 segregating sit
es (pi = 0.082%) were identified within 1,089 bp of the ZFX sequence in a s
ample of 336 X chromosomes. Four of these polymorphisms, which contributed
most of the diversity, were located within an Alu insert disrupting the ZFY
-ZFX homology ( pi(Alu) = 0.24%). The diversity in the homologous portion o
f the ZFX intron, although higher than that in ZEY, was lower than that fou
nd in genomic segments believed to evolve neutrally; interspecies divergenc
e in both segments was also reduced. Although this suggests that the evolut
ion of both ZFY and ZFX homologs may not be entirely neutral, both Tajima a
nd HKA tests did not reject neutrality. The lack of statistical significanc
e may be attributed to a lack of power in these tests (the low divergence a
nd variability values reduce the power of the HKA and Tajima tests, respect
ively); furthermore, Homo sapiens has recently undergone a rapid population
growth, and selection is more difficult to detect in an expanding populati
on. Therefore, the failure to reject neutrality does not necessarily indica
te the absence of selection. In this context, the phylogenetic argument was
given more weight in our interpretations. The high level of sequence ident
ity in ZFY and ZFX segments, in spite of their separation 80-130 MYA, refle
cts a lower mutation rate as compared with other segments believed to under
go unconstrained evolution. Thus, the possibility of weak selection contrib
uting to the low level of nucleotide diversity in the last ZFY intron canno
t be excluded and should be kept in mind in the population genetics studies
based on Y chromosome variability.