Glucocorticoid receptor-induced down-regulation of MMP-9 by ginseng components, PD and PT contributes to inhibition of the invasive capacity of HT1080 human fibrosarcoma cells
Mt. Park et al., Glucocorticoid receptor-induced down-regulation of MMP-9 by ginseng components, PD and PT contributes to inhibition of the invasive capacity of HT1080 human fibrosarcoma cells, MOL CELLS, 9(5), 1999, pp. 476-483
We examined the effects of the purified ginseng components, panaxadiol (PD)
and panaxatriol (PT), on the expression of matrix metalloproteinase-9 (MMP
-9) in highly metastatic HT1080 human fibrosarcoma cell line. A significant
down-regulation of MMP-9 by PD and PT was detected by Northern blot analys
is. However, the expression of MMP-2 was not changed by treatment with PD a
nd PT. Quantitative gelatin based zymography confirmed a markedly reduced e
xpression of MMP-9, but not MMP-2 in the treatment of PD and PT, To investi
gate whether the reduced level of MMP-9 by PD and PT affects the invasive c
apacity of HT1080 cells, we conducted an ill vitro invasion assay with PD a
nd PT treated cells. The results of the in vitro invasion assay revealed th
at PD and PT reduced tumor cell invasion through a reconstituted basement m
embrane in the transwell chamber. Because of the similarity of chemical str
ucture between PD, PT and dexamethasone (Dexa), a synthetic glucocorticoid,
we investigated whether the down-regulation of MMP-9 by PD and PT were med
iated by the nuclear translocation of glucocorticoid receptor (GR), Increas
ed GR in the nucleus of HT1080 human fibrosarcoma cells treated by PD and P
T was detected by immunocytochemistry, Western blot and gel retardation ass
ays confirmed the increase of GR in the nucleus after treatment with PD and
PT. These results suggest that GR-induced down-regulation of MMP-9 by PD a
nd PT contributes to reduce the invasive capacity of HT1080 cells.