Refinement of the method for research on "Biosonics", sonic wave regulation of cell growth: Use of a turntable to balance the external effects such as oscillatory energies

Our environment is full of a variety of oscillations, or their pulses, whic h produce sound waves with different waveforms and frequencies. Cells such as bacteria are thought to survive and grow by accepting these sonic signal s, based on our several published and unpublished experiments. To more prec isely measure the effects of sonic signals on the functions of cells, it is important to balance the external effects such as oscillatory energies, wh ich are converted to sonic signals that it radiate the experimental system and promote and sustain the growth of bacteria. In this communication an improved method for measuring the response of cell s to the growth-promoting and/or growth-sustaining effects of sound waves i s described. This improve ment was done by placing the Petri dishes contain ing the cell cultures on a slowly rotating turntable in a thermoregulated, ventilated incubator. This not only excluded unbalanced background values a mong cultures, but also resulted in markedly low background values in zero- control experiments that omitted sonic signal donor materials. Moreover, sh ielding the Petri dishes with aluminum foil or iron plates and rotating the cultures resulted in almost complete discrimination of the external effect s such as oscillatory energies.