Studies on proteolysis of hemoglobin by Alcalase 0.6L are described. Trials
were conducted in a batch reactor at different operating conditions. Degre
es of hydrolysis in the proteolysates were determined and kinetics of the r
eaction was considered in detail in relation to substrate concentration, en
zyme concentration, temperature, degree of hydrolysis and reaction time. Fr
om the results a general kinetic equation for the enzymic hydrolysis of pro
teins is suggested. The proposed kinetic model allows an easier determinati
on of kinetic parameters using only a few experiments. (C) 1999 Elsevier Sc
ience Ltd. All rights reserved.