Modeling of enzymatic protein hydrolysis

Studies on proteolysis of hemoglobin by Alcalase 0.6L are described. Trials were conducted in a batch reactor at different operating conditions. Degre es of hydrolysis in the proteolysates were determined and kinetics of the r eaction was considered in detail in relation to substrate concentration, en zyme concentration, temperature, degree of hydrolysis and reaction time. Fr om the results a general kinetic equation for the enzymic hydrolysis of pro teins is suggested. The proposed kinetic model allows an easier determinati on of kinetic parameters using only a few experiments. (C) 1999 Elsevier Sc ience Ltd. All rights reserved.