N. Andarwulan et K. Shetty, Improvement of pea (Pisum sativum) seed vigour response by fish protein hydrolysates in combination with acetyl salicylic acid, PROCESS BIO, 35(1-2), 1999, pp. 159-165
Acetyl salicylic acid (ASA) and fish protein hydrolysate (FPH) were used fo
r stimulation of vigour of pea (Pisum sativum) seeds. Pea seeds were pre-hy
drated in water which contained 50 mu M of ASA, 2 ml 1(-1) of FPH (standard
ized mackerel hydrolysates) and 2 ml 1(-1) of a combination of ASA and FPH
(ASA/FPH) for 24 h and followed by germination in potting soil for 5 and 10
days. After 5 and 10 days, the average plant height and weight increased i
n plants with FPH and ASA/FPH treatments, but decreased slightly in plants
with ASA treatment. After 10 days, the average plant height with FPH and AS
A/FPH treatments increased by 9% (23.3 mm) and 11% (28.8 mm) respectively,
while the average fresh weight of seedling increased by 15% (13 mg) for bot
h treatments, respectively. The average phenolics content and guaiacol pero
xidase (GPX) activity of pea seedling increased following ASA and FPH treat
ment alone on day 5. Antioxidant activity in all pea seedlings was high on
day 10 compared to day 5. On either day there were no difference in antioxi
dant activity between treatments. Glucose-6-phosphate dehydrogenase (G-6-PD
H) activity and free proline content were higher on day 5 than on day 10. T
he average G-6-PDH activity in pea seedling treated with FPH and ASA/FPH wa
s higher than ASA and control treatments. The plant response to ASA/FPH was
better than FPH treatment alone. These results suggest that combination of
50 mu M of ASA and FPH with the concentration of 2 ml 1(-1) (ASA/FPH) can
enhance seed performance of peas according to some criteria of seedling vig
our and thus have potential for use as a seed treatment. (C) 1999 Elsevier
Science Ltd. All rights reserved.