Electrospray ionization mass spectrometry of 5-methyl-2 '-deoxycytidine and its determination in urine by liquid chromatography/electrospray ionization tandem mass spectrometry

The behaviour of 5-methyl-2'-deoxycytidine (m(5)dCyd, claimed to be a poten tial marker for leukaemia) during the electrospray process was studied. In particular, considerations concerning the effect of solution chemistry (e.g . analyte concentration, pH, etc.) on electrospray ionization mass spectra were drawn. Furthermore, a procedure using liquid chromatography/electrospr ay ionization tandem mass spectrometry (LC/ESI-MS/MS) for the determination of urinary m(5)dCyd is described, The method Is simple, sensitive and high ly specific. The pre-treatment procedure gave an average recovery of 79% (r elative standard deviation (RSD) of 3%), Method performance was evaluated o n spiked urine samples covering the concentration range from 50 ng/mL to 10 mu g/mL, the same as that of an existing inhibition ELISA method. Contrary to findings based on this immunoassay technique, urinary m(5)dCyd in healt hy individuals was not detectable and did not increase in the presence of t he malignant disease. Copyright (C) 1999 John Wiley & Sons, Ltd.