M. Toraason et al., Oxidative stress acid DNA damage in Fischer rats following acute exposure to trichloroethylene or perchloroethylene, TOXICOLOGY, 138(1), 1999, pp. 43-53
Oxidative DNA damage is emerging as an biomarker of effect in studies asses
sing the health risks of occupational chemicals. Trichloroethylene (TCE) an
d perchloroethylene (PERC) are used in the dry cleaning industry and their
metabolism can produce reactive oxygen compounds. The present study examine
d the potential for TCE and PERC to induce oxidative DNA damage in rats tha
t was detectable as increased urinary excretion of 8-hydroxydeoxyguanosine
(8OHdG). Thiobarbaturic acid reactive substances (TBARS) and 8-epi-prostagl
andin F-2 alpha (8epiPGF) were also measured as biomarkers of increased oxi
dative stress. Male Fischer rats were administered a single i.p. injection
of 0, 100, 500, or 1000 mg/kg of PERC or TCE. Control rats received only ve
hicle (1:4 v/v of Alkamuls/water). A positive control group received 100 mg
/kg 2-nitropropane (2NP). Rats were sacrificed 24 h after dosing. In rats r
eceiving 2NP or TCE but not PERC, TEARS and the 8OHdG/dG ratios were signif
icantly elevated in liver. Lymphocyte 8OHdG/dG was not affected significant
ly by 2NP, TCE or PERC. In rats receiving 2NP, urinary excretion of 8OHdG a
nd 8epiPGF2 were significantly increased. In rats receiving TCE or PERC, si
gnificant increases in 8epiPGF2 or 8OHdG were not evident. Results indicate
that a single high dose of TCE, but not PERC, can induce an increase in ox
idative DNA damage in rat liver. However, the usefulness of 8OHdG as a biom
arker of TCE-induced oxidative DNA damage is questionable. (C) 1999 Publish
ed by Elsevier Science Ireland Ltd. All rights reserved.