Y. Kitasako et al., Light and scanning electron microscopy of the inner surfaces of resins used in direct pulp capping, AM J DENT, 12(5), 1999, pp. 217-221
Purpose: To investigate with the scanning electron microscopy (SEM) the inn
er surface of the resin restorative used as a direct pulp capping, Material
s and Methods: Class V cavities were prepared on the facial surfaces of 90
intact monkey teeth, and the pulps were intentionally exposed with a carbid
e bur through the cavity floor. Each exposed pulp was capped with one of th
ree commercially available adhesive resin systems (BondWell LC, GC; Imperva
Bond, Shofu; Clearfil Liner Bond II, Kuraray). The cavities were restored
with an adhesive resin and a hybrid resin-based composite. Histopathologica
l changes of the mechanically exposed pulp were evaluated at 3, 30 and 60 d
ays postoperatively. The interface between the resin and pulp tissues on th
e inner surface of the resin restorative was observed by SEM. Results: Bact
erial penetration along the cavity walls and pulp tissue could not be detec
ted in any specimen. Slight inflammatory cell infiltration was the main ini
tial reaction, while dentin bridge formation was the major long-term reacti
on in all groups. The resin surface adjacent to the etched cavity floor con
tained numerous resin tags corresponding to the dentin tubules. Remnants of
pulpo-dentin complex, such as fragments of blood clots, pulpal cell, and d
entin were also seen scattered along the inner resin surface.