M. Yoshimoto et al., MYCN gene amplification - Identification of cell populations containing double minutes and homogeneously staining regions in neuroblastoma tumors, AM J PATH, 155(5), 1999, pp. 1439-1443
Citations number
35
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Neuroblastoma is the second most common solid tumor occurring in children.
Amplification of the MYCN oncogene is associated with poor prognosis. To id
entify neuroblastoma tumors with MYCN amplification, we studied the number
of copies of MYCN in interphase cells by fluorescence in situ hybridization
in 20 neuroblastoma patients. MYCN amplification appeared in 7 tumor speci
mens. Interphase and metaphase studies showed a tumor cell population with
both forms of amplification, double minutes and homogeneously staining regi
ons, in two patients. These patients showed a smaller tumor cell subpopulat
ion with the presence of more than one homogeneously staining region, sugge
sting that gene amplification was undergoing karyotype evolution.