Adsorptive and electrochemical behaviors of estradiol valerate at a mercury electrode

It was found that estradiol valerate could be adsorbed at a mercury electro de under open circuit. The adsorptive and electrochemical behaviors of estr adiol valerate on a static mercury electrode were investigated by cyclic vo ltammetry, linear scan voltammetry and chronocoulometry. Based on this, a s ensitive and selective adsorptive stripping square-wave voltammetric method was developed for the determination of estradiol valerate based on the opt imization of solution conditions and electrochemical parameters. It was fou nd that in a Britton-Robinson buffer solution containing 18% alcohol (pH 9. 5), estradiol valerate gave a sensitive reductive peak at potential -1.29 V (vs. SCE) and the peak current was linear with the concentration of estrad iol valerate in the range 2.0 x 10(-8)-2.5 x 10(-6) mol L-1. The detection limit was 1.1 x 10(-8) mol L-1. The interference of some common steroid est rogens was examined and it was found that they did not interfere in the det ermination of estradiol valerate in the present system.