GM-CSF stimulates proliferation of clonal leukemic bone marrow cells in acute myeloid leukemia (AML) in vitro

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is known to stimu late granulocytes, monocytes, and macrophages. We studied the effect of GM- CSF on (clonal) bone marrow (BM) cells obtained from AML patients after 7 d ays of culture in vitro: BM samples were obtained from 19 AML patients at d iagnosis (DIA), from two patients with persisting disease (PERS), from eigh t patients in complete remission (CR), and from 12 healthy donors. Flow-cyt ometric comparison of differentiated, CD 15-positive cells or of CD34-posit ive blast cells before and after cultivation showed that the proportion of CD15-positive cells was increased in nine of 12 healthy BM samples, in 14 o f 19 cases at DIA, in one of three cases during PERS, and in five of six ca ses in CR of AML. The proportion of CD34-positive cells was increased in on e of 12 healthy BM samples, in seven of 19 cases at DIA, in one of two case s during PERS, and in three of seven cases in CR of AML, Southern blot anal ysis (SBA) performed in six cases during the course of AML, before and afte r cell culture, showed that clonal DNA increased after GM-CSF treatment in three of five cases studied at DIA, in six of nine cases studied in CR, in the one case studied at PERS, and in the one studied at relapse (REL). In o ne case of trisomy 8 at DIA a normal karyotype was demonstrated in CR, Howe ver, after 7 days of cultivation of the cells in GM-CSF the trisomy 8 was d etected in two of 17 metaphases isolated from colony-cells from methylcellu lose cultures. Our data show that a 7-day treatment of BM cells with GM-CSF induced a differentiation of healthy and leukemic BM cells in the great ma jority of cases. An enrichment of CD34-positive cells was not achieved in h ealthy BM samples. However, in 70% of the cases in CR and in 30% of the cas es at DIA of AML, clonal CD34-positive cells were enriched. This means that GM-CSF stimulates ('primes') leukemic cell growth in vitro.