Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells ofStreptomyces clavuligerus NP1

Authors
Fernandez, MJ Adrio, JL Piret, JM Wolfe, S Ro, S Demain, AL
Citation
Mj. Fernandez et al., Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells ofStreptomyces clavuligerus NP1, APPL MICR B, 52(4), 1999, pp. 484-488
Citations number
12
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
ISSN journal
01757598 → ACNP
Volume
52
Issue
4
Year of publication
1999
Pages
484 - 488
Database
ISI
SICI code
0175-7598(199910)52:4<484:SEOGIT>2.0.ZU;2-L
Abstract
Growth of Streptomyces clavuligerus NP1 in the presence of methanol or etha nol resulted in a marked increase in production of cephalosporin(s) from pe nicillin G by resting cells. The mycelium produced in alcohol-supplemented medium was fragmented and dispersed as compared with growth in control medi um. HPLC analysis showed that at least two products were present in the bio transformation supernatant fluid after 1 h incubation. One of them has been identified as deacetoxycephalosporin G (DAOG).