Isolation and characterization of viridin, a new 65 kDa antifungal proteinfrom the mould Trichoderma viride

A new extracellular antifungal protein with a yield of 10 mg per liter was isolated from the culture medium of the mould Trichoderma viride, The prote in, which we named viridin, was purified by carboxymethyl-cellulose cation- exchange chromatography and Superose 12 HR 10/30 high-performance liquid ch romatography, Viridin, a basic protein of approximately 65 kDa as determine d by SDS-PAGE, inhibits the growth of the cotton pathogen Verticillum dahli ae, the IC50 being 6 mu M.