Micro-perfusion flow cell for imaging cultured cells

Authors
Agnihotri, N Kisaalita, WS Keith, CH
Citation
N. Agnihotri et al., Micro-perfusion flow cell for imaging cultured cells, BIOTECHNIQU, 27(4), 1999, pp. 722
Citations number
14
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOTECHNIQUES
ISSN journal
07366205 → ACNP
Volume
27
Issue
4
Year of publication
1999
Database
ISI
SICI code
0736-6205(199910)27:4<722:MFCFIC>2.0.ZU;2-J
Abstract
We present a unique design for a flow cell with a small working volume that allows rapid displacement of media viewed under high power and short worki ng distance objectives. The flow cell has a small internal depth (ca. 0.033 cm) and volume (ca. 0.05 mL) and is easy to handle. Made of Delrin(R), the flow cell is biologically inert. We have used the flow cell for fluorescen ce imaging of PC12 cells loaded with tetramethylrhodamine dextran (TMRD) an d other dyes.