We have investigated prospectively the incidence of bacterial contamination
of 114 spinal and 20 epidural needles collected immediately after lumbar p
uncture of the subarachnoid or epidural space. Bacteriological examination
revealed bacterial contamination of 24 (17.9%) of the needles, mainly coagu
lase-negative staphylococci (21; 15.7%) followed by yeasts (2; 1.5%), enter
ococcus (1; 0.8%), pneumococcus (1; 0.8%) and micrococcus (1; 0.8%). Our re
sults suggest that even during aseptic puncture for lumbar anaesthesia, the
re is a significant rate of needle contamination.