The accurate measurement of ammonium concentrations is fundamental to under
standing nitrogen biogeochemistry in aquatic ecosystems. Unfortunately, the
commonly used indophenol blue method often yields inconsistent results, pa
rticularly when ammonium concentrations are low. Here, we present a fluorom
etric method that gives precise measurements of ammonium over a wide range
of concentrations and salinities emphasizing submicromolar levels. The proc
edure not only solves analytical problems but also substantially simplifies
sample collection and preservation. It uses a single working reagent (cons
isting of orthophthaldialdehyde, sodium sulfite, and sodium borate) that is
stable for months when stored in the dark. The working reagent and sample
can be mixed immediately after sample collection and the reaction proceeds
to completion within 3 h at room temperature. Matrix effects and background
fluorescence can be corrected without introducing substantial error. This
simple method produces highly reproducible results even at very low ammoniu
m concentrations.