Enantiomer separation of 7-des-methyl-ormeloxifene using sulfated beta-cyclodextrin in countercurrent chromatography

The enantiomers of 7-des-methyl-ormeloxifene were separated by countercurre nt chromatography (CCC) using sulfated beta-cyclodextrin as chiral selector , representing the first reported successful application of a cyclodextrin derivative in CCC-based resolutions. The choice of chiral selector relies o n extreme separation factors observed in chiral capillary electrophoresis, and suitable CCC conditions were developed employing an analytical toroidal coil countercurrent chromatograph. Preparative separation of the enantiome rs was performed using a conventional, preparative CCC-instrument. Chiralit y 11:768-771, 1999. (C) 1999 Wiley-Liss, Inc.