H. Takahashi et al., Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease, CL DIAG LAB, 6(6), 1999, pp. 983-985
We established four hybridoma cell lines producing monoclonal antibodies (M
Abs) against 14-3-3 proteins. Immunoblot analysis revealed that epsilon and
gamma isoforms were specifically increased in premortem cerebrospinal flui
d samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermor
e, dot immunoblot analysis shelved that MAbs were more specific for native
antigen than polyclonal antibodies were.