Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease

Authors
Takahashi, H Iwata, T Kitagawa, Y Takahashi, RH Sato, Y Wakabayashi, H Takashima, M Kido, H Nagashima, K Kenney, K Gibbs, CJ Kurata, T
Citation
H. Takahashi et al., Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease, CL DIAG LAB, 6(6), 1999, pp. 983-985
Citations number
12
Categorie Soggetti
Immunology
Journal title
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
ISSN journal
1071412X → ACNP
Volume
6
Issue
6
Year of publication
1999
Pages
983 - 985
Database
ISI
SICI code
1071-412X(199911)6:6<983:ILOEAG>2.0.ZU;2-#
Abstract
We established four hybridoma cell lines producing monoclonal antibodies (M Abs) against 14-3-3 proteins. Immunoblot analysis revealed that epsilon and gamma isoforms were specifically increased in premortem cerebrospinal flui d samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermor e, dot immunoblot analysis shelved that MAbs were more specific for native antigen than polyclonal antibodies were.