From 1992-1998, Burkholderia pseudomallei was isolated from only 9 (0.25%)
of 3653 cultures of blood from febrile patients admitted to the Centre for
Tropical Diseases in Ho Chi Minh City, an infectious disease referral cente
r for southern Vietnam. Soil was sampled from 407 sites in 147 rice fields
along the 5 major roads radiating from Ho Chi Minh City. B, pseudomallei wa
s isolated from 73 sites (18%) in 39 rice fields (27%), but only 15 (21%) o
f the 71 isolates from 9 (6%) of 147 fields were the virulent L-arabinose (
ara)-negative biotype, All except 1 of the fields with the ara-negative bio
type were close to the homes of the patients with melioidosis, The low inci
dence of melioidosis in the provinces around Ho Chi Minh City may be explai
ned by the restricted distribution of ara-negative B. pseudomallei in the s
oil in this area.