W. Haedicke et al., Oligoclonal expansions of T-cell repertoire in gastric mucosa associated lymphoid tissue type B-cell lymphoma and adjacent gastritis, DIAGN MOL P, 8(3), 1999, pp. 138-144
Citations number
36
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Local stimulation by Helicobacter pylori (HP), autoantigen: and a concurren
t T-cell-mediated stimulation of B cells are believed to play an important
role in gastric mucosa-associated lymphoid tissue (MALT) type B cell lympho
magenesis. Many autoimmune diseases have shown to lead to a skewed T-cell r
epertoire with autoantigen specific expansions and deletions. Characterizat
ion of lymphoma and gastritis areas of seven gastrectomy specimens using a
T-cell receptor beta variable chain (TCR beta V) family-specific reverse tr
anscriptase (RT)polymerase chain reaction (PCR) assay and fluorescence-acti
vated cell sorter (FACS) analysis revealed a local chronic and acute activa
tion of T cells in lymphoma and an oligoclonal T-cell repertoire in gastrit
is and in lymphoma, partially sharing the same clones. Local activation and
a partial identity suggest that an antigenic challenge caused by a common
local pathogen may still continue to take place in MALT type lymphoma as in
gastritis, consistent with the view that gastritis may be a precursor lesi
on of MALT type lymphoma. Expansions that were found only in one of the com
partments suggest that also an immune hyperstimulation may contribute to th
e T-cell repertoire, possibly because of certain tissue antigens. Deletions
of TCR PV families found only in gastritis underline the view that autoant
igen may play an important role in its pathogenesis.