Catalytic subunit of telomerase expression is related to RNA component expression

Citation
G. Stanta et al., Catalytic subunit of telomerase expression is related to RNA component expression, FEBS LETTER, 460(2), 1999, pp. 285-288
Citations number
25
Categorie Soggetti
Biochemistry & Biophysics
Journal title
FEBS LETTERS
ISSN journal
00145793 → ACNP
Volume
460
Issue
2
Year of publication
1999
Pages
285 - 288
Database
ISI
SICI code
0014-5793(19991029)460:2<285:CSOTEI>2.0.ZU;2-R
Abstract
Telomere length is maintained by the ribonucleoprotein enzyme telomerase. T he RNA component of telomerase (hTR) is widespread, and only the expression of the mRNA encoding the catalytic protein subunit (hTRT) is correlated wi th telomerase activity. We have studied the level of expression of hTR and hTRT in four different models of neoplastic and preneoplastic lesions using the RT-PCR method on RNA extracted from paraffin-embedded human tissues af ter microdissection, The expression at the mRNA level was compared with the enzymatic activity. Our results suggest that there may be a reciprocal con trol at the transcriptional level of the expression of hTRT and hTR which i n turn is associated with tumor progression. (C) 1999 Federation of Europea n Biochemical Societies.