Indirect fluorimetric detection of cyclodextrins via postcolumn mixing with 2-p-toluidinyl-6-naphthalenesulfonate in liquid chromatography

Authors
Takeuchi, T Miwa, T
Citation
T. Takeuchi et T. Miwa, Indirect fluorimetric detection of cyclodextrins via postcolumn mixing with 2-p-toluidinyl-6-naphthalenesulfonate in liquid chromatography, HRC-J HIGH, 22(11), 1999, pp. 609-612
Citations number
18
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
HRC-JOURNAL OF HIGH RESOLUTION CHROMATOGRAPHY
ISSN journal
09356304 → ACNP
Volume
22
Issue
11
Year of publication
1999
Pages
609 - 612
Database
ISI
SICI code
0935-6304(199911)22:11<609:IFDOCV>2.0.ZU;2-G
Abstract
Cyclodextrins (CD's) were visualized by postcolumn mixing with 2-p-toluidin ylnaphthalene sulfonate (TNS) in liquid chromatography. The indirect detect ion is based on fluorescence enhancement due to the formation of an inclusi on complex between TNS and CD's. beta-CD gave larger signal intensity than alpha- and gamma-CD. Different selectivities were observed for alkyl-bonded silica stationary phases with different chain length.