Mouse monoclonal antibodies against Phytolacca americana antiviral proteinPAP I

Authors
Kaloyanova, D Kyurkchiev, S Xu, J Abouhaidar, M Ivanov, I
Citation
D. Kaloyanova et al., Mouse monoclonal antibodies against Phytolacca americana antiviral proteinPAP I, HYBRIDOMA, 18(4), 1999, pp. 367-370
Citations number
13
Categorie Soggetti
Immunology
Journal title
HYBRIDOMA
ISSN journal
0272457X → ACNP
Volume
18
Issue
4
Year of publication
1999
Pages
367 - 370
Database
ISI
SICI code
0272-457X(199908)18:4<367:MMAAPA>2.0.ZU;2-V
Abstract
Four hybridoma lines are constructed producing monoclonal antibodies agains t the pokeweed (Phytolacca americana) antiviral protein PAP I. Two of the a ntibodies, 4E8 and 5D3, are characterized in more detail. They recognize am ino acid sequences rather than conformational changes and their epitopes ar e 65% distinct. One of these antibodies (5D3) is used to study localization of recombinant PAP I in Escherichia coil cells by immuno-gold electron mic roscopy.