Amperometric biosensor based on deoxyribonucleic acid and platinum(II) complexes: New analytical capabilities

Authors
Babkina, SS Ulakhovich, NA Medyantseva, EP Zyavkina, YI
Citation
Ss. Babkina et al., Amperometric biosensor based on deoxyribonucleic acid and platinum(II) complexes: New analytical capabilities, J ANALYT CH, 54(11), 1999, pp. 1070-1075
Citations number
30
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF ANALYTICAL CHEMISTRY
ISSN journal
10619348 → ACNP
Volume
54
Issue
11
Year of publication
1999
Pages
1070 - 1075
Database
ISI
SICI code
1061-9348(199911)54:11<1070:ABBODA>2.0.ZU;2-F
Abstract
A new method for the immobilization of DNA on a nitrocellulose membrane was proposed. The activity of immobilized biomolecules is retained their activ ity. The immobilized DNA was used as the biosensitive part of an amperometr ic sensor based on a stationary mercury-film electrode. To extend the analy tical capabilities of the sensor, the peak current of catalytic hydrogen ev olution at -1.4 V was examined (this peak current occurs in the complexatio n of platinum(II) with antibodies to DNA). The biosensor was used for deter mining antibodies to DNA in the concentration range 5.0 x 10(-10)-7.0 x 10( -8) M. An immunoassay procedure was proposed.