D. Walker et al., Structural abnormalities do not explain the early functional abnormalitiesin the peripheral nerves of the streptozotocin diabetic rat, J ANAT, 195, 1999, pp. 419-427
The streptozotocin (STZ)-diabetic rat, the most commonly employed model of
experimental diabetic neuropathy, is characterised by a reduction in nerve
conduction velocity, pain threshold and blood flow. Whether or not structur
al abnormalities underlie these functional abnormalities is unclear. 10 adu
lt male Sprague-Dawley STZ-diabetic rats (diabetes duration 27 d) and 10 ag
e-matched (23 wk) control animals were studied. Motor nerve conduction velo
city (m s(-1)) was significantly reduced in diabetic (41.31+/-0.8) compared
with control (46.15+/-1.5) animals (P < 0.001). The concentration of sciat
ic nerve glucose (P < 0.001), fructose (P < 0.001) and sorbitol (P < 0.001)
was elevated, and myoinositol (P < 0.001) was reduced in diabetic compared
with control animals. Detailed morphometric studies demonstrated no signif
icant difference in fascicular area, myelinated fibre density, fibre and ax
on areas as well as unmyelinated fibre density and diameter. Endoneurial ca
pillary density, basement membrane area and endothelial cell profile number
did not differ between diabetic and control animals. However, luminal area
(P < 0.03) was increased and endothelial cell area (P < 0.08) was decrease
d in the diabetic rats. We conclude there is no detectable structural basis
for the reduction in nerve conduction velocity, pain threshold or blood fl
ow, observed in the streptozotocin diabetic rat.