New approach for determination of macroscopic binding constants of ligandsto macromolecules

A new method has been developed for determining macroscopic binding constan ts of a few surfactants to some macromolecules. Then, the binding of ionic surfactants (cetyltrimethyl ammonium bromide, tetradecyltrimethyl ammonium bromide, and sodium tetradecyl sulfate) [STS]) to macromolecules (bovine se rum albumin, human serum albumin, and polyvinyl pyrollidone was investigate d. The binding constants and binding isotherms were calculated using this n ew method. Experimental data were obtained through a potentiometry techniqu e. Finally, the results obtained were compared with the results obtained ba sed on the Wyman binding potential concept. (C) 1999 Academic Press.