This study was aimed at determining the ability of different coronal tempor
ary fillings to prevent corono-apical penetration of bacteria. A total of 1
03 human teeth, including three controls, were instrumented, obturated with
gutta-percha, coronally sealed with either Cavit, Intermediate Restorative
Material (IRM), glass-ionomer cement, Cavit/glass-ionomer cement, or IRM/g
lass-ionomer cement, respectively. Each root was fixed with wax between two
chambers: the coronal chamber harboring soy broth with 10(8) colony-formin
g units of Streptococcus mutans/ml, the apical chamber containing sterile s
oy broth. The latter was checked daily for turbidity, indicating corono-api
cal penetration of bacteria. The Cavit group, the IRM group, and the Cavit/
glass-ionomer cement group showed significantly more leakage than the glass
-ionomer cement group of the IRM/glass-ionomer cement group. If a sample le
aked, all except one (IRM/glass-ionomer cement) leaked before-day 12. This
in vitro study seems to indicate that only glass-ionomer cement and IRM com
bined with glass-ionomer cement may prevent bacterial penetration to the pe
riapex of root-filled teeth over a 1-month period.