Leukocyte recruitment in the cerebrospinal fluid of mice with experimentalmeningitis is inhibited by an antibody to junctional adhesion molecule (JAM)

Citation
A. Del Maschio et al., Leukocyte recruitment in the cerebrospinal fluid of mice with experimentalmeningitis is inhibited by an antibody to junctional adhesion molecule (JAM), J EXP MED, 190(9), 1999, pp. 1351-1356
Citations number
34
Categorie Soggetti
Medical Research General Topics
Journal title
JOURNAL OF EXPERIMENTAL MEDICINE
ISSN journal
00221007 → ACNP
Volume
190
Issue
9
Year of publication
1999
Pages
1351 - 1356
Database
ISI
SICI code
0022-1007(19991101)190:9<1351:LRITCF>2.0.ZU;2-H
Abstract
The mechanisms that govern leukocyte transmigration through the endothelium are not yet fully defined. Junctional adhesion molecule (JAM) is a newly c loned member of the immunoglobulin superfamily which is selectively concent rated at tight junctions of endothelial and epithelial cells. A blocking mo noclonal antibody (BV11 mAb) directed to JAM was able to inhibit monocyte t ransmigration through endothelial cells in in vitro and in vivo chemotaxis assays. In this study, we report that BV11 administration was able to atten uate cytokine-induced meningitis in mice. The intravenous injection of BV11 mAb significantly inhibited leukocyte accumulation in the cerebrospinal fl uid and infiltration in the brain parenchyma. Blood-brain barrier permeabil ity was also reduced by the mAb. We conclude that JAM may be a new target i n limiting the inflammatory response that accompanies meningitis.