Survival of Escherichia coli O157 : H7 in ground-beef patties during storage at 2, -2, 15 and then -2 degrees C, and -20 degrees C

The survival of Escherichia coli O157:H7 and of a nonpathogenic control str ain of E. coli was monitored in raw ground beef that was stored at 2 degree s C for 4 weeks, -2 degrees C for 4 weeks, 15 degrees C for 4 h and then -2 degrees C for 4 weeks, and -20 degrees C. Irradiated ground beef was inocu lated with one E. coli control strain or with a four-strain cocktail of E. coli O157:H7 (ca. 10(5) CFU/g), formed into patties (30 to 45 g), and store d at the appropriate temperature. The numbers of the E. coli control strain decreased by 1.4 log(10) CFU/g, and pathogen numbers declined 1.9 log(10) CFU/g when patties were stored for 4 weeks at 2 degrees C. When patties wer e stored at -2 degrees C for 4 weeks, the numbers of the E. coli control st rain and the serotype O157:H7 strains decreased 2.8 and 1.5 log(10) CFU/g, respectively. Patties stored at 15 degrees C for 4 h prior to storage at -2 degrees C for 4 weeks resulted in 1.6 and 2.7 log(10)-CFU/g reduction in t he numbers of E. coli and E. coli O157:H7, respectively. Storage of retail ground beef at 15 degrees C for 4 h (tempering) did not result in increased numbers of colony forming units per gram, as determined with violet red bi le, MRS lactobacilli, and plate-count agars. Frozen storage (-20 degrees C) of ground-beef patties that had been inoculated with a single strain of E. coli resulted in approximately a 1 to 2 log(10)-CFU/g reduction in the num bers of the control strain and individual serotype O157:H7 strains after 1 year. There was no significant difference between the survival of the contr ol strain and the O157:H7 strains, nor was there a difference between O157: H7 strains. These data demonstrate that tempering of ground-beef patties pr ior to low-temperature storage accelerated the decline in the numbers of E. coli O157:H7.