Differential modulation of human epidermal Langerhans cell maturation bg ultraviolet B radiation

Citation
S. Nakagawa et al., Differential modulation of human epidermal Langerhans cell maturation bg ultraviolet B radiation, J IMMUNOL, 163(10), 1999, pp. 5192-5200
Citations number
63
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
00221767 → ACNP
Volume
163
Issue
10
Year of publication
1999
Pages
5192 - 5200
Database
ISI
SICI code
0022-1767(19991115)163:10<5192:DMOHEL>2.0.ZU;2-J
Abstract
UVB irradiation of the skin causes immunosuppression and Ag-specific tolera nce in which Langerhans cells (LC) are involved. We tested the effect of UV B on LC that had migrated out of cultured epidermal sheets derived from the skin that was irradiated es vivo (200, 400, 800, or 1600 J/m(2)). Two sepa rate subpopulations of LC were distinguished: large-sized LC with high HLA- DR expression, and HLA-DR-low, small LC, UVB stimulated the maturation of t he former LC subset as demonstrated by enhanced up-regulation of CD80, CD86 , CD54, CD40, and CD83 and reduced CD1a expression in comparison with unirr adiated controls. In contrast, the latter LC exhibited little or no up-regu lation of these molecules except for high CD1a expression and high binding of annexin V, indicating that they were apoptotic, although their CD95 expr ession was relatively low. Stimulation of enriched LC with CD40 ligand-tran sfected cells and IFN-gamma revealed that the release of IL-1 beta, IL-6, I L-8, and TNF-alpha was enhanced by UVB, In comparison with HLA-DR-low LC, H LA-DR-high LC were the principal IL-8 producers as demonstrated by intracel lular cytokine staining, and they retained more accessory function. There w as no detectable secretion of IL-12 p70, and IL-18 production was neither a ffected by any stimulus nor by UVB. These results suggest a dual action of UVB on LC when irradiated in situ: 1) immunosuppression by preventing matur ation and inducing apoptotic cell death in part of LC, and 2) immunopotenti ation by enhancing the up-regulation of costimulatory molecules and the pro duction of preinflammatory cytokines in another part.