Anti-CD43 monoclonal antibody L11 flocks migration of T cells to inflamed pancreatic islets and prevents development of diabetes in nonobese diabeticmice
Gg. Johnson et al., Anti-CD43 monoclonal antibody L11 flocks migration of T cells to inflamed pancreatic islets and prevents development of diabetes in nonobese diabeticmice, J IMMUNOL, 163(10), 1999, pp. 5678-5685
Nonobese diabetic mice are a well-known model for human insulin-dependent d
iabetes mellitus. These mice develop autoimmune-mediated inflammation of th
e pancreatic islets, followed by destruction of the insulin-producing beta
cells and development of diabetes. Nonobese diabetic mice also have salivar
y gland inflammation, and serve as a model for human Sjogren's syndrome. T
cells are a prominent component of the inflammatory infiltrate in these sit
es, and T cell recruitment from the blood is thought to be essential for th
e initiation and maintenance of pathologic tissue damage. A unique mAb to m
urine CD43, L11, has recently been shown to block the migration of T cells
from blood into organized lymphoid tissues. Here we demonstrate that L11 si
gnificantly inhibits T cell migration from blood into inflamed islets and s
alivary glands, Treatment of nonobese diabetic mice with L11 from 1 to 4 or
8 to 12 wk of age led to significant protection against the development of
diabetes. Moreover, protection was long-lived, with decreased incidence of
diabetes even months after cessation of Ab administration. When treatment
was started at 1 wk of age, L11 inhibited the development of inflammation i
n pancreatic islets and salivary glands. L11 treatment had no long-term eff
ect on numbers or phenotypes of peripheral lymphocytes, These data indicate
that anti-CD43 Abs that block T cell migration may be useful agents for th
e prevention or treatment of autoimmune diseases including insulin-dependen
t diabetes mellitus and Sjogren's syndrome.