Transverse-relaxation-optimized (TROSY) gradient-enhanced triple-resonanceNMR spectroscopy

Authors
Loria, JP Rance, M Palmer, AG
Citation
Jp. Loria et al., Transverse-relaxation-optimized (TROSY) gradient-enhanced triple-resonanceNMR spectroscopy, J MAGN RES, 141(1), 1999, pp. 180-184
Citations number
26
Categorie Soggetti
Chemistry & Analysis","Physical Chemistry/Chemical Physics
Journal title
JOURNAL OF MAGNETIC RESONANCE
ISSN journal
10907807 → ACNP
Volume
141
Issue
1
Year of publication
1999
Pages
180 - 184
Database
ISI
SICI code
1090-7807(199911)141:1<180:T(GT>2.0.ZU;2-N
Abstract
Two modifications to sensitivity-enhanced gradient-selected TROSY-based tri ple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-fli pback solvent suppression. The modifications are illustrated for the HNCO-T ROSY experiment, but are applicable to all triple-resonance experiments tha t detect proton magnetization after a reverse polarization transfer step fr om a N-15 spin. The methods are applied to yeast triosephosphate isomerase, a symmetric dimer with 248 amino acid residues per monomer, (C) 1999 Acade mic Press.