Selectivity paradigm in lipase reactions: Correlating actual and observed regioselectivity in hydrolysis of unsymmetrical diacetates

The first simple method using labeling technique has been developed to conf irm the actual and observed selectivities in the enzymatic hydrolysis of un symmetrical diacetates and to measure the enzyme selectivity efficiency. Th e simple method consists of enzymatic hydrolysis of unsymmetrical diacetate followed by labeling of the hydroxyacetate formed! with. CD3COOD/DCC and e nzymatic rehydrolysis of the labeled compound under the identical set of re action conditions to estimate the amount of label retained by H-1 NMR spect roscopy. The amount of label lost directly indicates the extent of regiosel ective action of the enzyme.