The lipoxygenase pathway is involved in elicitor-induced phytoalexin accumulation in plane tree (Platanus acerifolia) cell-suspension cultures

The Ceratocystis fimbriata f.sp. platani 66 kDa glycoprotein elicitor-induc ed secretion of soluble coumarins by plane tree (Platanus acerifolia (Aiton ) Wild) cell-suspension cultures was investigated by studying the possible involvement of the octadecanoid pathway in the cell response. When cell-sus pension cultures were treated with the glycoprotein elicitor, the cells exh ibited a rapid and transient increase in lipoxygenase activity, in synthesi s of endogenous jasmonic acid prior to the accumulation of coumarin phytoal exins. The treatment of cells with an inhibitor of lipoxygenase (ETYA) befo re elicitor addition, drastically reduced the lipoxygenase activity, the pr oduction of endogenous jasmonic acid and phytoalexin accumulation. The resu lts demonstrate the role of the jasmonate pathway in the intracellular sign al cascade.