Automated DNA fragment analysis by high performance ultrathin-layer agarose gel electrophoresis

Ultrathin-layer agarose gel electrophoresis combines the advantages of the multilane format of conventional slab and the high performance capabilities of capillary gel electrophoresis. This article describes the detection of separated sample components in realtime by using an integrated scanning las er-induced fluorescence-avalanche photodiode detection system. Ultrathin-la yer agarose gel electrophoresis is a novel and high performance separation technique that provides a simple platform for fast deoxyribonucleic acid fr agment analysis with the advantage that the same gel-filled cassette can be used simultaneously for a size range of 20-23,130 base pairs. The author p rovides examples of rapid genotyping and high-throughput mutation analysis of polymerase chain reaction-generated fragments.