Immunohistochemical detection of PGL-1, LAM, 30 kD and 65 kD antigens in leprosy infected paraffin preserved skin and nerve sections

A panel of lipid, carbohydrate and protein antibodies were optimized for us e in detecting M. leprae antigens in paraffin embedded material. Skin and n erve biopsies from 13 patients across the leprosy spectrum were studied. Al l antibodies detected antigen in tissues with a BI >1. Phenolic-glycolipid was not detected in bacteriologically negative tissue but lipoarabinomanan (LAM) and protein antigens were detected. Staining with LAM was strongest a nd gave least background. The transfer of this immunohistochemical techniqu e to paraffin embedded material will allow examination of tissue with bette r morphology and from clinics without access to tissue freezing facilities.